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anti smo  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti smo
    Anti Smo, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+smo/Smoothened+Antibody+(3D10)/pm41898701-387-78-79
    Average 94 stars, based on 1 article reviews
    anti smo - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: 7-Ketocholesterol and cholestane-triol increase expression of SMO and LXRα signaling pathways in a human breast cancer cell line
    Article Snippet: After washing with DPBS, cells were permeabilized with 0.1% Triton X-100 solution (93443 Sigma Aldrich) at 4 °C for 15 min, followed by blocking with 5% BSA (A9418 Sigma Aldrich) for 40 min at room temperature. .. Cells were incubated overnight with the following antibodies: anti-SMO (1:100 dilution; NBP2–24543-Novus Biologicals, CO, USA); anti-SHh (1:400 dilution; ab53281- Abcam, Cambridge, UK); anti-LXRα (1:50 dilution; ab41902 Abcam); anti-ABCA1 (1:50 dilution; NB100–2068- Novus Biologicals); and anti-ABCG1 (1:50 dilution; H9619-M03- Abnova, Taipei, Taiwan). .. After incubating with anti-SMO and anti-SHh antibodies, cells were incubated for 1 h more with the anti-rabbit secondary antibody, AlexaFluor 488 (1:1000 dilution; A-11008- Molecular Probes, OR, USA).

    Article Title: 7-Ketocholesterol Links Sterol Homeostasis to Hedgehog Signaling and Stress–Survival Responses in MSCs from Patients with Acute Myeloid Leukemia
    Article Snippet: .. MSCs were seeded in 96-well plates, treated for 24 h, fixed with 4% paraformaldehyde, permeabilized (0.1% Triton X-100), blocked (5% BSA), and incubated overnight at 4 °C with primary antibodies against lipid transporters/sensors and signaling proteins using the following sources and dilutions: anti-ABCA1 (Novus Biologicals, Centennial, CO, USA, 1:200), anti-ABCC1 (Abcam, Cambridge, UK, 1:50), anti-ABCC2 (Abcam, 1:50), anti-ABCD4 (Novus Biologicals, 1:500), anti-ABCG1 (Novus Biologicals, 1:100), anti-ABCG2 (Novus Biologicals, 1:250), anti-LRP (Abcam, 1:100), anti-CD147 (Abcam, 1:100), anti-SHH (Abcam, 1:400), anti-SMO (Novus Biologicals, 1:100), anti-GLI3 (Novus Biologicals, 1:100), anti-LXRα (Abcam, 1:50), anti-LXRβ (Sigma-Aldrich, St. Louis, MO, USA, 1:200), anti-PPARγ (Novus Biologicals, 1:200), anti-caveolin-1 (Abcam, 1:500), and anti-survivin (Abcam, 1:500). ..

    Article Title: 7-Ketocholesterol Links Sterol Homeostasis to Hedgehog Signaling and Stress-Survival Responses in MSCs from Patients with Acute Myeloid Leukemia.
    Article Snippet: .. MSCs were seeded in 96-well plates, treated for 24 h, fixed with 4% paraformaldehyde, permeabilized (0.1% Triton X-100), blocked (5% BSA), and incubated overnight at 4 ◦C with primary antibodies against lipid transporters/sensors and signaling proteins using the following sources and dilutions: anti-ABCA1 (Novus Biologicals, Centennial, CO, USA, 1:200), anti-ABCC1 (Abcam, Cambridge, UK, 1:50), anti-ABCC2 (Abcam, 1:50), anti-ABCD4 (Novus Biologicals, 1:500), anti-ABCG1 (Novus Biologicals, 1:100), anti-ABCG2 (Novus Biologicals, 1:250), anti-LRP (Abcam, 1:100), anti-CD147 (Abcam, 1:100), anti-SHH (Abcam, 1:400), anti-SMO (Novus Biologicals, 1:100), anti-GLI3 (Novus Biologicals, 1:100), antiLXRα (Abcam, 1:50), anti-LXRβ (Sigma-Aldrich, St. Louis, MO, USA, 1:200), anti-PPARγ (Novus Biologicals, 1:200), anti-caveolin-1 (Abcam, 1:500), and anti-survivin (Abcam, 1:500). ..



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    (A-C) Schematic diagrams of GFP:Smo, Cherry:Smo, and GFP:Cherry:Smo fusion proteins and smo 3 wings from flies grown at 25°C with BAC-GFP:Smo (A’), BAC-Cherry:Smo (B’), and BAC-GFP:Cherry:Smo (C’) transgenes. (D) Wing disc from wild type fly stained <t>with</t> <t>α-Smo</t> antibody. Intensity plot of area bounded by green dashed lines below, with location of compartment border indicated. (E-H”) Frontal optical section images of discs with genotypes GFP:Smo/Cherry:Smo (E,G) and GFP:CherrySmo/+ (F,H) acquired with GFP (Green) or RFP (Red) channel. (E-F”) Fixed and stained with a-GFP or a-RFP antibodies. (G-H”) Unfixed, live fluorescence.
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    (A-C) Schematic diagrams of GFP:Smo, Cherry:Smo, and GFP:Cherry:Smo fusion proteins and smo 3 wings from flies grown at 25°C with BAC-GFP:Smo (A’), BAC-Cherry:Smo (B’), and BAC-GFP:Cherry:Smo (C’) transgenes. (D) Wing disc from wild type fly stained <t>with</t> <t>α-Smo</t> antibody. Intensity plot of area bounded by green dashed lines below, with location of compartment border indicated. (E-H”) Frontal optical section images of discs with genotypes GFP:Smo/Cherry:Smo (E,G) and GFP:CherrySmo/+ (F,H) acquired with GFP (Green) or RFP (Red) channel. (E-F”) Fixed and stained with a-GFP or a-RFP antibodies. (G-H”) Unfixed, live fluorescence.
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    (A-C) Schematic diagrams of GFP:Smo, Cherry:Smo, and GFP:Cherry:Smo fusion proteins and smo 3 wings from flies grown at 25°C with BAC-GFP:Smo (A’), BAC-Cherry:Smo (B’), and BAC-GFP:Cherry:Smo (C’) transgenes. (D) Wing disc from wild type fly stained <t>with</t> <t>α-Smo</t> antibody. Intensity plot of area bounded by green dashed lines below, with location of compartment border indicated. (E-H”) Frontal optical section images of discs with genotypes GFP:Smo/Cherry:Smo (E,G) and GFP:CherrySmo/+ (F,H) acquired with GFP (Green) or RFP (Red) channel. (E-F”) Fixed and stained with a-GFP or a-RFP antibodies. (G-H”) Unfixed, live fluorescence.
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    Image Search Results


    (A-C) Schematic diagrams of GFP:Smo, Cherry:Smo, and GFP:Cherry:Smo fusion proteins and smo 3 wings from flies grown at 25°C with BAC-GFP:Smo (A’), BAC-Cherry:Smo (B’), and BAC-GFP:Cherry:Smo (C’) transgenes. (D) Wing disc from wild type fly stained with α-Smo antibody. Intensity plot of area bounded by green dashed lines below, with location of compartment border indicated. (E-H”) Frontal optical section images of discs with genotypes GFP:Smo/Cherry:Smo (E,G) and GFP:CherrySmo/+ (F,H) acquired with GFP (Green) or RFP (Red) channel. (E-F”) Fixed and stained with a-GFP or a-RFP antibodies. (G-H”) Unfixed, live fluorescence.

    Journal: bioRxiv

    Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

    doi: 10.64898/2026.01.08.698469

    Figure Lengend Snippet: (A-C) Schematic diagrams of GFP:Smo, Cherry:Smo, and GFP:Cherry:Smo fusion proteins and smo 3 wings from flies grown at 25°C with BAC-GFP:Smo (A’), BAC-Cherry:Smo (B’), and BAC-GFP:Cherry:Smo (C’) transgenes. (D) Wing disc from wild type fly stained with α-Smo antibody. Intensity plot of area bounded by green dashed lines below, with location of compartment border indicated. (E-H”) Frontal optical section images of discs with genotypes GFP:Smo/Cherry:Smo (E,G) and GFP:CherrySmo/+ (F,H) acquired with GFP (Green) or RFP (Red) channel. (E-F”) Fixed and stained with a-GFP or a-RFP antibodies. (G-H”) Unfixed, live fluorescence.

    Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

    Techniques: Staining, Fluorescence

    (A-C) Sagittal views of wing discs stained with (A,A’) α-Smo (gray), α-Ptc (green), and phalloidin (blue), or (B,B’) expressing BAC-GFP:Smo (green), BAC-Cherry:Smo (red), and mIFP ( ptc-Gal4>mIFP; Smo:GFP:Cherry/+ ); dashed lines indicate apical surface. (C-G) Wing disc expressing BAC-GFP:Smo (green) and Cad:Cherry; (C) sagittal, (D-G) frontal views in A compartment, P compartment and in apical or basolateral sections. (H-L) Same as (C-G), except wing discs express Cad:GFP (green) and BAC-encoded Cherry:Smo (red).

    Journal: bioRxiv

    Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

    doi: 10.64898/2026.01.08.698469

    Figure Lengend Snippet: (A-C) Sagittal views of wing discs stained with (A,A’) α-Smo (gray), α-Ptc (green), and phalloidin (blue), or (B,B’) expressing BAC-GFP:Smo (green), BAC-Cherry:Smo (red), and mIFP ( ptc-Gal4>mIFP; Smo:GFP:Cherry/+ ); dashed lines indicate apical surface. (C-G) Wing disc expressing BAC-GFP:Smo (green) and Cad:Cherry; (C) sagittal, (D-G) frontal views in A compartment, P compartment and in apical or basolateral sections. (H-L) Same as (C-G), except wing discs express Cad:GFP (green) and BAC-encoded Cherry:Smo (red).

    Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

    Techniques: Staining, Expressing

    Wild-type wing disc stained with α-Smo (red) and α-Ptc (green) at apical (A,A’) and basolateral (B,B’) optical planes. (A,B) Smo and Ptc staining; (A’,B’) Smo staining only. (A”,B”) Graphs showing intensity plots of images in (A,B) across A/P axis. (C,D) Same as (A,B) for unfixed disc expressing BAC-encoded GFP:Smo and mIFP driven by ptc-Gal4 showing fluorescence of Smo (green) and mIFP (blue) ( ptc-Gal4>mIFP ; GFP: Smo / + ). (E,F) Same as (C,D) for disc expressing BAC-encoded Cherry:Smo (red) and mIFP driven by ptc-Gal4 ( ptc-Gal4>mIFP ; Cherry : Smo / + ). (G) Same as (F) showing membrane-localized fluorescence only.

    Journal: bioRxiv

    Article Title: Smoothened turnover regulated by Hedgehog signaling in Drosophila

    doi: 10.64898/2026.01.08.698469

    Figure Lengend Snippet: Wild-type wing disc stained with α-Smo (red) and α-Ptc (green) at apical (A,A’) and basolateral (B,B’) optical planes. (A,B) Smo and Ptc staining; (A’,B’) Smo staining only. (A”,B”) Graphs showing intensity plots of images in (A,B) across A/P axis. (C,D) Same as (A,B) for unfixed disc expressing BAC-encoded GFP:Smo and mIFP driven by ptc-Gal4 showing fluorescence of Smo (green) and mIFP (blue) ( ptc-Gal4>mIFP ; GFP: Smo / + ). (E,F) Same as (C,D) for disc expressing BAC-encoded Cherry:Smo (red) and mIFP driven by ptc-Gal4 ( ptc-Gal4>mIFP ; Cherry : Smo / + ). (G) Same as (F) showing membrane-localized fluorescence only.

    Article Snippet: The following antibodies were used: mouse α-GFP (Roche), rabbit α-RFP (Rockland), mouse α-Ptc (DSHB, Apa1), mouse α-En (DSHB, 4D9), DAPI, Phalloidin, mouse α-Dlg1, (DSHB, 4F3), α-Rab7 , α-Smo (DSHB, 20C6), Alexa633 conjugated-Phalloidin (Invitrogen), DAPI (Invitrogen), AbN ( ).

    Techniques: Staining, Expressing, Fluorescence, Membrane